独特抗原识别模式，复宏汉霖H药临床前研究及结构分析发表于PLOS ONE
H药斯鲁利单抗是复宏汉霖自主研发的人源化IgG4型抗PD-1单抗，可增强人体T细胞的肿瘤杀伤功能。体外研究显示斯鲁利单抗可增强T细胞的活化和增殖，在体内多种肿瘤模型中也展现出显著的抗肿瘤活性，并在非小细胞肺癌（NSCLC）和三阴性乳腺癌（TNBC）肿瘤模型中分别与Nivolumab和Pembrolizumab表现出相似的抑瘤效果。同时，斯鲁利单抗与贝伐珠单抗联合使用可解除PD-1/PD-L1的免疫抑制信号并抑制血管生成，在结直肠癌（CRC）和NSCLC肿瘤模型中展现出显著的抑瘤效果，为斯鲁利单抗和贝伐珠单抗联合治疗临床试验的开展奠定了基础。为了进一步探究斯鲁利单抗与PD-1的结合机制，研究者对斯鲁利单抗Fab段与PD-1复合物的晶体结构进行了解析。斯鲁利单抗与Pembrolizumab具有更相近的结合表位，两者表现出相反的重链(HC)和轻链(LC)结合方式，斯鲁利单抗的结合表面面积为445Å2（占PD-1表面的55%）。
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关于复宏汉霖
复宏汉霖前瞻性布局了一个多元化、高质量的产品管线，涵盖20多种创新单克隆抗体，并全面推进基于自有抗PD-1单抗斯鲁利单抗的肿瘤免疫联合疗法。继国内首个生物类似药汉利康®（利妥昔单抗）、中国首个自主研发的中欧双批单抗药物汉曲优®（曲妥珠单抗，欧盟商品名：Zercepac®）、汉达远®（阿达木单抗）和汉贝泰®（贝伐珠单抗）相继获批上市，创新产品斯鲁利单抗MSI-H实体瘤的上市注册申请已纳入优先审评审批程序，HLX01利妥昔单抗类风湿关节炎新适应症、斯鲁利单抗鳞状非小细胞肺癌适应症的上市注册申请也正在审评中。有限公司亦同步操作就15个产品设备、九个免疫性携手冶疗策划方案在世界各国标准内开发20单项临床研究试验报告，境内外受权周到遍布欧美国家趋势生物工程药贸易市场上和有很多兴盛贸易市场上。
The preclinical and structural analysis results of Henlius’ innovative anti-PD-1 mAb serplulimab, published in an international journal PLOS ONE
Recently, the preclinical study results and molecular characteristics of Henlius’ innovative anti-PD-1 monoclonal antibody (mAb) serplulimab (HLX10) were published in PLOS ONE. The results showed that compared to nivolumab and pembrolizumab, serplulimab showed similar or better bioactivity in vitro and in vivo and anti-tumor activity in several tumour models. Furthermore, serplulimab synergizes with bevacizumab biosimilar 汉贝泰 (HLX04) to promote robust tumour activity. Detailed epitope of the antigen-binding fragment (Fab) of serplulimab in complex with PD-1 analysis showed that serplulimab has a unique mode of recognition compared to the clinically approved PD-1 antibodies pembrolizumab and nivolumab with a similar binding epitope region to pembrolizumab.
Serplulimab, a fully humanized IgG4 mAb against PD-1 receptor, increased functional activities of human T-cells. The in vitro study showed that serplulimab activates T-cell proliferation and activization. The in vivo study showed anti-tumour efficacy in several tumour models. Serplulimab showed anti-tumour efficacy similar to nivolumab in non-small cell lung cancer (NSCLC) tumour model and pembrolizumab in triple-negative breast cancer (TNBC) tumour model. The combined inhibition of PD-1/PD-L1 and angiogenesis pathways using bevacizumab HLX04 may enhance a sustained suppression of cancer-related angiogenesis and tumour elimination, and serplulimab showed favorable tumour suppressive efficacy both in colorectal cancer (CRC) and NSCLC tumour models. The results laid foundation to the clinical trials of serplulimab in combination with HLX04. To gain insight into how serplulimab achieves PD-1 recognition, scientists determined the co-crystal structure of the Fab of serplulimab in complex with PD-1 and compared this structure to the previously determined structures of pembrolizumab and nivolumab. Compared to the clinically approved PD-1 antibodies pembrolizumab and nivolumab, serplulimab has a similar binding epitope region to pembrolizumab. Serplulimab and pembrolizumab showed an opposite heavy chain (HC) and light chain (LC) usage. Serplulimab interface occupies a solvent-accessible overlapping surface area of 445Å2 (55% of PD-L1 surface) on PD-1.
AboutPLOS ONE
PLOS is a nonprofit, Open Access publisher empowering researchers to accelerate progress in science and medicine by leading a transformation in research communication. PLOS ONE is an inclusive journal community working together to advance science for the benefit of society, now and in the future. Founded with the aim of accelerating the pace of scientific advancement and demonstrating its value, PLOS ONE believes all rigorous science needs to be published and discoverable, widely disseminated and freely accessible to all. The research of PLOS ONE publish is multidisciplinary and, often, interdisciplinary. PLOS ONE accepts research in over two hundred subject areas across science, engineering, medicine, and the related social sciences and humanities. PLOS ONE evaluates submitted manuscripts on the basis of methodological rigor and high ethical standards.
About Henlius
Henlius (2696.HK) is a global biopharmaceutical company with the vision to offer high-quality, affordable and innovative biologic medicines for patients worldwide with a focus on oncology, autoimmune diseases and ophthalmic diseases. Up to date, 4 products have been launched in China, 1 in the European Union (EU), 3 New Drug Applications (NDAs) accepted for review in China. Since its inception in 2010, Henlius has built an integrated biopharmaceutical platform with core capabilities of high-efficiency and innovation embedded throughout the whole product life cycle including R&D, manufacturing and commercialisation. It has established global R&D centres and a Shanghai-based manufacturing facility certificated by China and the EU Good Manufacturing Practice (GMP).
Henlius has pro-actively built a diversified and high-quality product pipeline covering over 20 innovative monoclonal antibodies (mAbs) and has continued to explore immuno-oncology combination therapies with proprietary serplulimab (anti-PD-1 mAb) as backbone. Apart from the launched products 汉利康® (rituximab), the first China-developed biosimilar, 汉曲优® (trastuzumab, Zercepac® in the EU), the first China-developed mAb biosimilar approved both in China and in the EU, 汉达远® (adalimumab) and 汉贝泰® (bevacizumab), the NDA of innovative product serplulimab indicated for MSI-H solid tumors has been granted priority review, and the NDAs of HLX01 (rituximab) for the treatment of rheumatoid arthritis and serplulimab for the treatment of squamous non-small cell lung cancer are also under review. What's more, Henlius has conducted over 20 clinical studies for 11 products and 8 combination therapies worldwide, expanding its presence in major market as well as emerging market.
联系方式
校园媒体：PR@Henlius.com
投入资金者：IR@Henlius.com
【符合期刊论文】
Issafras H, Fan S, Tseng C-L, Cheng Y, Lin P, Xiao L, et al. (2021) Structural basis of HLX10 PD-1 receptor recognition, a promising anti-PD-1 antibody clinical candidate for cancer immunotherapy. PLoS ONE 16(12): e0257972. //doi.org/10.1371/journal.pone.0257972
